Granzyme B人酶聯(lián)免疫斑點(diǎn)法（ELISPOT）試劑盒
產(chǎn)品名稱： Granzyme B人酶聯(lián)免疫斑點(diǎn)法（ELISPOT）試劑盒
英文名稱： C11 CGL1 CSPB CTLA1 Fragmentin 2 Granzyme 2 SECT
產(chǎn)品編號(hào)： ab46617
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 英國
品牌商標(biāo)： abcam
更新時(shí)間： null
使用范圍： null
- 聯(lián)系人 :
- 地址 : 深圳市寶安區(qū)西鄉(xiāng)寶民二路賢基大廈4E
- 郵編 :
- 所在區(qū)域 : 廣東
- 電話 : 133****4454 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : 1484332550@qq.com
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- 檢測方法Colorimetric
- 測試
5 x 96 well plate
- 樣品類型
Cell culture supernatant, Cell culture extracts
- 檢測類型Sandwich (qualitative)
- 實(shí)驗(yàn)步驟Multiple steps standard assay
- 種屬反應(yīng)性
與反應(yīng):?Human
產(chǎn)品概述
This ELISPOT assay is designed to enumerate Granzyme B producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing Granzyme B production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of Granzuyme B producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
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The Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
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Principle of Method
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After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
組件
5 x 96 tests
10 x 96 tests
| 96 PVDF-bottomed-well plates. | 5 units | 10 units |
| Bovine Serum albumin | 1 x 1g | 2 x 1g |
| Dry skimmed milk | 1 x 1g | 2 x 1g |
| Granzyme B Biotinylated detection antibody | 1 vial | 2 vials |
| Granzyme B Capture Antibody | 1 x 500μl | 2 x 500μl |
| Ready-to-use BCIP/NBT substrate buffer | 1 x 50ml | 2 x 50ml |
| Streptavidin - Alkaline Phosphatase conjugated | 1 x 50μl | 2 x 50μl |
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