Shh (Mouse) Recombinant Protein
產品名稱： Shh (Mouse) Recombinant Protein
英文名稱： Shh (Mouse) Recombinant Protein
產品編號： P4621
產品價格： 0
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍： null
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse Shh (Q15465) recombinant protein expressed in Escherichia coli.
- Sequence:
- MIIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
- Theoretical MW (kDa):
- 19.8
- Form:
- Lyophilized
- Preparation Method:
- Escherichia coli expression system
- Endotoxin Level:
- < 0.01 ng/ug or < 0.1 EU/ug.
- Activity:
- The activity is determined by the dose-dependent increase of alkaline phosphatase activity in C3H/10T1/2 fibroblasts (CCL-226). The expected ED50 for this effect is 0.48-0.72 ug/mL.
- Storage Buffer:
- Lyophilized with 10 mM Na2PO4, pH 7.5.
- Storage Instruction:
- Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- Result of activity analysis
-
Serial dilutions of mouse Shh, starting at 5 ug/mL, were added to with CCL-226 cells in the presence of 1 uM Retinoic Acid. Alkaline phosphatase was measured and the linear portion of the curve was us used to calculate the ED50.
- Application Image
- Functional Study
- SDS-PAGE