重組人DNAM-1蛋白（Recombinant Human DNAM-1 Protein），GMP級

產品貨號：GMP-TL718

產品描述（Description）

DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

分子別名（Synonym）

DNAM1、CD226、PTA1

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列編碼人DNAM-1（NCBI Reference Sequence: NP_006557.2）表達帶有His標簽在C末端

純度（Purity）

≥95%采用 SDS-PAGE 凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

分子量（Molecular Weight）

重組人DNAM-1蛋白 包含229個氨基酸，預測理論分子量為27.7 kD

生物活性（Biological Activity）

固定人 DNAM-1(His Tag)5ug/mL(100uL/孔) 能夠結合 CD155(Fc Tag)的線性范圍是 5-80ng/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1、DNAM-1 controls NK cell activation via an ITT-like motif Zhanguang Zhang, Ning Wu, Yan Lu, Dominique Davidson, Marco Colonna, André Veillette J Exp Med. 2015 Nov 16; 212(12): 2165–2182. doi: 10.1084/jem.20150792 

2、Expression of CD226 is associated to but not required for NK cell education Arnika K. Wagner, Nadir Kadri, Johanna Sn?ll, Petter Brodin, Susan Gilfillan, Marco Colonna, Günter Bernhardt, Petter H?glund, Klas K?rre, Benedict J. Chambers Nat Commun. 2017; 8: 15627. Published online 2017 May 31. doi: 10.1038/ncomms15627 

3.、CD8 T cell defect of TNF-α and IL-2 in DNAM-1 deficient mice delays clearance in vivo of a persistent virus infection Megan J. Welch, John R. Teijaro, Hanna A. Lewicki, Marco Colonna, Michael B.A. Oldstone Virology. Author manuscript; available in PMC 2013 Aug 1.Published in final edited form as: Virology. 2012 Aug 1; 429(2): 163–170. Published online 2012 May 12. doi: 10.1016/j.virol.2012.04.006

  注意：For research and manufacturing use.