Anti-Polyethylene glycol抗體[PEG-2-128]-抗體-抗體-生物在線
深圳市宇德立生物科技有限公司
Anti-Polyethylene glycol抗體[PEG-2-128]

Anti-Polyethylene glycol抗體[PEG-2-128]

商家詢價

產品名稱： Anti-Polyethylene glycol抗體[PEG-2-128]

英文名稱： PEG antibody

產品編號： ab133471

產品價格： null

產品產地： 英國

品牌商標： abcam

更新時間： null

使用范圍：

深圳市宇德立生物科技有限公司
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?

  • 經測試應用WB,?ELISAmore details
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to Polyethylene glycol 20K (PEG20K).

  • 表位This antibody recognizes the backbone of the Polyethylene glycol molecule.
  • 常規說明

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb??technology. RabMAb??technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.?

    ab133471 is useful in measuring the pharmacokinetics of Polyethylene glycol modified molecules. This antibody is specific to the backbone of PEG molecule. ?
    For detection of PEG?methoxy group, see?Anti-Polyethylene glycol antibody [PEG-B-47] - ab51257

    For PEG specific ELISA kit -?see?Polyethylene Glycol (PEG) RabMAb ELISA Kit - ab138914

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents.?
    Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
    when performing ELISA using anti-PEG products.

    Antibody Isotype: ?Please note, this RabMAb product's isotype is Rabbit IgM. ?Anti-IgM secondary is recommended as a secondary antibody.?
    List of anti-Rabbit IgM secondaries.

    Learn more about PEG RabMAb primary antibodies and kit -?PEG RabMAb Product Portal Page

性能

  • 形式Liquid
  • 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存儲溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • ?
  • 純度Protein A purified
  • Primary antibody說明ab133471 is useful in measuring the pharmacokinetics of Polyethylene glycol modified molecules.
  • 克隆單克隆
  • 克隆編號PEG-2-128
  • 同種型IgM
  • 研究領域

應用

Our?Abpromise guarantee?covers the use of?ab133471?in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Abreviews 說明
WB ? 1/1000. The MW of the WB band for ab133471 varies based on the PEGylated molecule detected.
ELISA ? Use a concentration of 2 - 10 μg/ml.

靶標

  • 別名
    • PEG antibody

Anti-Polyethylene glycol antibody [PEG-2-128] 圖像

  • All lanes :?Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471) at 1/1000 dilution (purified)

    Lane 1 :?PEG20K
    Lane 2 :?MBP-PEG20K

    Lysates/proteins at 5 μg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgM, (H+L) at 1/500 dilution

    Blocking buffer: 15% BSA/TBS.

    Dilution buffer: 10% PAA /TBST.

  • Direct ELISA antigen dose-response curve using purified ab133471. Antigen concentration of 0-100 ng/mL. A HRP-conjugated goat anti-rabbit IgM (1/1000) was used as the secondary antibody.

  • Direct ELISA antigen dose-response curve using purified ab133471. Antigen concentration of 0-10000 ng/mL. A HRP-conjugated goat anti-rabbit IgM (1/1000) was used as the secondary antibody.

  • Sandwich ELISA antigen dose-response curve using purified ab133471.

    Capture antibody -?ab133471?(10000 ng/ml).

    Antigens - MBP-PEG20K and?ADI-PEG (0-1000 ng/ml).

    Detection -?ab53449?(500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin)?followed by peroxidase-conjugated streptravidin (1/1000).

  • All lanes :?Anti-Polyethylene glycol antibody [PEG-2-128] (ab133471) at 1/1000 dilution (unpurified)

    Lane 1 :?MBP-PEG20K at 1 μg
    Lane 2 :?MBP-PEG20K at 0.5 μg
    Lane 3 :?PEG20K at 1 μg
    Lane 4 :?PEG20K at 0.5 μg

    Secondary
    HRP-conjugated goat anti-rabbit IgM at 1/2000 dilution    Gel concentration: 4-20%

  • Various concentrations of Polyethylene glycol polymers with amine end groups were coated in 96 wells. 2 ug/ml of unpurified?ab133471 was added. After washing, samples were incubated with?1 ug/ml of HRP-conjugated anti-rabbit IgM.

  • Various concentrations of Polyethylene glycol 20K (PEG20K) conjugated MBP and branched Polyethylene glycol conjugated BSA were coated in 96 wells. 2 ug/ml of unpurified ab133471 was added. After washing, samples were incubated with?1 ug/ml of HRP-conjugated anti-rabbit IgM.

  • 10 ug/ml of unpurified ab133471 was coated into 96 wells. Serial dilution of Polyethylene glycol 20K (PEG20K) conjugated MBP and PEGylated ADI were added. After washing, 1 ug/ml of biotinylated anti-Polyethylene glycol antibody was added. Streptavidin-HRP was then used to develop the color.