form:powder.
technique(s):cell culture | mammalian: suitable.
components:glucose: low;phenol red: yes;L-glutamine: yes;HEPES: no;NaHCO3: no;sodium pyruvate: yes.
General description:Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.
Application:Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).