DCK polyclonal antibody
產品名稱： DCK polyclonal antibody
英文名稱： DCK polyclonal antibody
產品編號： PAB2020
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DCK.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DCK.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunoprecipitation (1:500-1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity.
Hartford CM, Duan S, Delaney SM, Mi S, Kistner EO, Lamba JK, Huang RS, Dolan ME.Blood. 2009 Mar 5;113(10):2145-53. Epub 2008 Dec 24.
- 2.
- Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants.
Lamba JK, Crews K, Pounds S, Schuetz EG, Gresham J, Gandhi V, Plunkett W, Rubnitz J, Ribeiro R.J Pharmacol Exp Ther. 2007 Dec;323(3):935-45. Epub 2007 Sep 12.
- Applications
- Western Blot (Tissue lysate)
- The DCK polyclonal antibody (Cat # PAB2020) is used in Western blot to detect DCK in mouse intestine tissue lysate.
- Immunoprecipitation
- Deoxycytidine kinase (DCK) immunoprecipitated from NCI-H460 cells with 7.5 ug (microgram) of DCK polyclonal antibody (Cat # PAB2020) using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and current protocols in Cell Biology, 1998, 7.2.1-7.2.21.
Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1 : 700 dilution of DCK polyclonal antibody (Cat # PAB2020).
Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit).
No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.
- Entrez GeneID:
- 1633
- Protein Accession#:
- NP_000779;P27707
- Gene Name:
- DCK
- Gene Alias:
- MGC117410,MGC138632
- Gene Description:
- deoxycytidine kinase
- Omim ID:
- 125450
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq
- Other Designations:
- -
- Gene Pathway