人MMP抗體陣列-膜(10 Targets)-抗體相關-抗體-生物在線

產品名稱：人MMP抗體陣列-膜(10 Targets)

英文名稱：人MMP抗體Array -膜(10 Targets) (ab134004)

產品編號： ab134004

產品價格： null

產品產地：英國

品牌商標： abcam

更新時間： null

使用范圍：

深圳市宇德立生物科技有限公司

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樣品類型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
檢測類型Semi-quantitative
種屬反應性
與反應:?Human
產品概述

ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.

?

Targets:?MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4

Learn more about membrane antibody arrays
經測試應用Multiplex Protein Detectionmore details

組件	1 x 4 Membranes	1 x 8 Membranes
1000X HRP-Conjugated Streptavidin	1 x 50μl	1 x 50μl
1X Blocking Buffer	1 x 25ml	1 x 50ml
20X Wash Buffer I	1 x 10ml	1 x 20ml
20X Wash Buffer II	1 x 10ml	1 x 20ml
2X Cell Lysis Buffer	1 x 10ml	1 x 20ml
8-Well Plastic Tray	1 unit	1 unit
Biotin-Conjugated Anti-Cytokines	2 vials	4 vials
Cytokine Antibody Array Membranes	4 units	8 units
Detection Buffer C	1 x 1.5ml	1 x 2.5ml
Detection Buffer D	1 x 1.5ml	1 x 2.5ml

Our?Abpromise guarantee?covers the use of?ab134004?in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用	Abreviews	說明
Multiplex Protein Detection	?	Use at an assay dependent concentration.

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.
Quantification of Human MMP Antibody Array

Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

143B cells (Human osteosarcoma) were seeded at 1x10⁶?cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate.

Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134004.
Quantification of Human MMP Antibody Array

Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 μg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)This image is courtesy of an anonymous customer

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