Size??100ug
Cat code??200270
Species Cross-reactivity??H,M
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??43kDa
Sensitivity??This antibody detects endogenous levels Pyruvate Dehydrogenase and does not cross-react with related proteins.
Immunogen??Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
UniProt Number??P08559
GeneBank ID??NM_000284
Subcellular location??Mitochondrion matrix
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??PDHA; PHE1A; PDHCE1A
Gene_symbol??PDHA; PHE1A; PDHCE1A
Summary??The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniPort summary  Function??The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Application Images :??
Western blot detection of Pyruvate Dehydrogenase(C-terminus) antibody in 293,1299,mEsc&Tc1 cell lysates using Pyruvate Dehydrogenase(C-terminus) antibody (1:1000 diluted).
Predicted band size: 43KDa
Observed band size: 43KDa.
Immunocytochemistry of HeLa cells using anti- Pyruvate Dehydrogenase (C-terminus) antibody diluted 1:150.