兔單克隆抗體Cleaved Caspase-3 (Asp175) (5A1E)
產品名稱： 兔單克隆抗體Cleaved Caspase-3 (Asp175) (5A1E)
英文名稱： Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
產品編號： 9664S
產品價格： null
產品產地： usa
品牌商標： cst
更新時間： null
使用范圍：
- 聯系人 :
- 地址 : 濟南市天橋區
- 郵編 : 2500
- 所在區域 : 山東
- 電話 : 187****5463 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 1024837510@qq.com
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Product Usage Information
| Application | Dilutions |
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry | 1:2000 |
| Immunofluorescence | 1:400 |
| Flow Cytometry | 1:6400 |
Storage:?Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
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Specificity / Sensitivity
Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.
Species Reactivity:?Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology:?Dog
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Background
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
1.??Fernandes-Alnemri, T. et al. (1994)?J Biol Chem?269, 30761-4.
2.??Nicholson, D.W. et al. (1995)?Nature?376, 37-43.
Data Sheets & Documentation
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