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目錄號 
                        
規(guī)格 
                        
價格 
                        
說明書下載 
                    
                    

                        
C082A
                        
2μg
                        
400元
                        

                    
                    

                        
C082B
                        
10μg
                        
1800元
                    
                    

                        
C082C
                        
50ug
                        
4500元
                    
                
            
            
        
        

            

            
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receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication.
            


            ·Description

            Recombinant rabbit tumor necrosis factor alpha is a non-glycosylated cytokine produced from E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17.4 Daltons. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
            


            ·Quality Control

            Biological activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 3.0 x 107 IU/mg. 

            Purity: Greater than 95% as determined by 

            (a) Analysis by SEC-HPLC. 

            (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

            Molecular weight: 17.3 KD+/-10% determined by reduced SDS-PAGE.

            Isoelectric Point: the main zone between 4.0~5.0 analysis by IEF.

            Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-.

            Endotoxin: Less than 0.1ng/μg (1 IEU/μg) as determined by LAL test. 

            

            ·Formulation

            Lyophilized from a 0.2 μm filtered solution in PBS. 

            

            ·Reconstitution

            It is recommended to reconstitute the lyophilized recombinant Rabbit TNF-alpha in PBS containing at least 0.1% human or bovine serum albumin. not less than 100μg/ml, which can then be further diluted to other aqueous solutions. 

            

            ·Storage

            Lyophilized samples are stable for up to twelve months from date of receipt at -20°C to -70°C. 

            

            Please avoid freeze-thaw cycles. 
            
        
        

            
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