PeproTech PeproGrow™hESC（胚胎干細胞）培養基-細胞/細菌培養-試劑-生物在線

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美國PeproTech(派普泰克)公司中國代表處
PeproTech PeproGrow™hESC（胚胎干細胞）培養基

PeproTech PeproGrow™hESC（胚胎干細胞）培養基

商家詢價

產品名稱： PeproTech PeproGrow™hESC（胚胎干細胞）培養基

英文名稱： PeproGrow™ hESC (Embryonic Stem Cell) Media

產品編號： PeproGrow- hESC

產品價格： 0

產品產地： 美國

品牌商標： PeproTech（派普泰克）

更新時間： null

使用范圍： null

美國PeproTech(派普泰克)公司中國代表處
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  • 地址 :  江蘇省蘇州市工業園區星湖街218號A1北座4樓L03
  • 郵編 : 215123
  • 所在區域 : 江蘇
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  • 郵箱 : peprotech.infochina@thermofisher.com

PeproGrow? hESC (Embryonic Stem Cell) Media

產品詳情

產品編號：hESC
產品描述：

PeproTech’s PeproGrow? hESC Media products are comprised of complete, chemicallydefined formulations designed for the feeder-free expansion and maintenance of both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). Intended for the culturing of hESCs and iPSCs in the undifferentiated, pluripotent state (Nanog+/Lin28+/Tra-1-60+/SSEA1-/SSEA4+/Oct4+), these formulations demonstrate less than 15% spontaneous differentiation as indicated by immunofluorescent staining and flow cytometry. These proprietary formulations were designed and developed by PeproTech in collaboration with the Stem Cell Training Course at Rutgers University. Each PeproGrow? hESC Medium Kit includes a bottle of basal medium and a separate, lyophilized component of PeproTech’s recombinant growth factors. Additional companion products are available separately and include the Animal-Free Human Vitronectin Matrix and Buffer Kit (catalog number AF-VMB-220), and the Cell Passaging/Non-Enzymatic Detachment Buffer (catalog number CPD-125). PeproTech’s Animal-Free Human Vitronectin Matrix and Buffer Kit can be used following either a surface-coating or premix method.


PeproGrow? hESC Medium Kit is an insulin-free formulation, in which insulin is replaced by a unique activator of similar pathways to eliminate possible interference during the measurement of insulin-production in subsequently differentiated cells.




Human Embryonic Stem Cell Basal Media and Growth Factor



PSC Immunophenotypes Are Maintained in PeproGrow hESC Medium


Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)?expanded in PeproGrow? hESC Medium maintain an?Oct4+/SSEA4+/Nanog+/Tra-1-60+/SSEA1- phenotype?as shown by?flow cytometry (A) and immunofluorescent microscopy (B).?


A.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?B.

Flow Cytometry Human ESC MediumImmunofluorescent Microscopy hESC Medium



Pluripotent Stem Cells Grow and Proliferate Rapidly in PeproGrow? hESC Medium

H1 Human ES Cells transduced with turboGFP-Neo driven by an Oct4 promoter and expanded on Matrigel proliferate rapidly when grown in PeproGrow? hESC Medium.

hESC Rapid Proliferation

Note:The manual for this media can be found?here

Cross Reactivity:
Human

Country Of Origin:?USA

Not for human use.