All Names: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Form of Antibody: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability: Store at -20℃/1 year?
Clonality: Monoclonal?
Immunogen: The antiserum was produced against Full-length β-Actin protein of human.
Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity/Sensitivity: β-Actin Mouse Monoclonal antibody detects endogenous levels of total β-Actin protein.
Reactivity: Human; Monkey; Mouse; Rat; hamster; Dog; Spodoptera frugiperda; chicken，rabbit
Applications: WB: 1:5,000-10,000 IHC: 1:200 IF: 1:200
The optimal working dilution should be determined by the end user.
References: Ponte P., Nucleic Acids Res. 12:1687-1696(1984).
Hillier L.W., Nature 424:157-164(2003).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
?

WB:Western blot analysis of extracts from Hela cell lysate(lane1) , Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3), using β-Actin Mouse Monoclonal antibody.