胞質(zhì)分裂專一蛋白7抗體說明書
產(chǎn)品名稱: 胞質(zhì)分裂專一蛋白7抗體說明書
英文名稱: 電詢
產(chǎn)品編號(hào): YM-H16451-X
產(chǎn)品價(jià)格: null
產(chǎn)品產(chǎn)地: 上海
品牌商標(biāo): 上海遠(yuǎn)慕
更新時(shí)間: null
使用范圍: 科研實(shí)驗(yàn)
- 聯(lián)系人 :
- 地址 : 上海浦東新區(qū)川沙經(jīng)濟(jì)園區(qū)
- 郵編 : 201200
- 所在區(qū)域 : 上海
- 電話 : 133****2040 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : m13310162040@163.com
"胞質(zhì)分裂專一蛋白7抗體說明書遠(yuǎn)慕生物長(zhǎng)期供應(yīng)免疫組化抗體、WB抗體、免疫組化試劑盒和抗體試驗(yàn)所需全部相關(guān)試劑、熒光標(biāo)記抗體、ELISA試劑盒、抗體、單克隆抗體、多克隆抗體、各種標(biāo)記的二抗IgG/IgM/IgD/IgA等科研實(shí)驗(yàn)抗體。產(chǎn)品名稱:胞質(zhì)分裂專一蛋白7抗體說明書
產(chǎn)品貨號(hào):YM-H16451-X
濃 度: 1mg/1ml
貯 存: 貯存于-20℃.
亞 型: IgG
性 狀:Lyophilized or Liquid
產(chǎn)品類型: 一抗 多抗體
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
胞質(zhì)分裂專一蛋白7抗體說明書產(chǎn)品WB實(shí)驗(yàn)中的對(duì)照系統(tǒng):
he chromogenic reaction (Note: two anti generally have two kinds ofcommonly used marker enzyme, HRP and AP, chromogenic substrate kit andits corresponding to DAB and BCIP/NBT, the general use ofchemiluminescence lysate.) Westernbloting is the first to transfer afterelectrophoresis separation of proteins from the gel onto a NC membrane,there are generally two ways: capillary blotting and electrophoreticblotting. Westernbloting is a common method used to detect the protein,through the immune reaction of antigen antibody can be quantitativelymeasured the relative molecular weight of antibody, and the relativeabundance of known antigen or antibody and the relation between. Atpresent, it is applied in many fields, detection of infectious diseasesand autoimmune diseases, allergies, etc. in clinic, in antibodyproduction field it with ELISA, ICC, IHC, IFA and other immunetechnology has become the main means of engaging a screening ofmonoclonal antibody production.
WB對(duì)照系統(tǒng)
陽性對(duì)照:最好有標(biāo)準(zhǔn)品,或陽性血清、陽性上清。
陰性對(duì)照:測(cè)血時(shí)用相應(yīng)小鼠未免疫血清,測(cè)培養(yǎng)上清,用無克隆培養(yǎng)上清。
空白對(duì)照:不加一抗,用1%BSA代替。
無關(guān)對(duì)照(替代對(duì)照):用無關(guān)項(xiàng)目一抗,或無關(guān)項(xiàng)目的抗體。
The principle of immunohistochemical method:
1. Immunofluorescence cytochemistry technology
2. Immune enzyme cytochemistry technology: within the cell or tissue on the corresponding antigen to locate or qualitative research.
3. Immune colloidal gold technique: with colloidal gold mark is A resistance, the combination of the two can resist or other specific immunoglobulin molecules (such as staphylococcus protein A) as A probe to qualitative tissue or cell antigen, positioning and quantitative research.
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胞質(zhì)分裂專一蛋白7抗體說明書
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