Product: Anti-ASK1 (CT), Polyclonal
Cat. No: PC-174 (100 μg)
Description:
Mitogen-activated protein (MAP) kinase
cascades are activated in response to various
extracellular stimuli, including cytokines, growth
factors and environmental stresses. A novel
MAP kinase kinase kinase (MAPKKK) was
recently identified and designated ASK1 (for
apoptosis signal-regulating kinase 1) and
MAPKKK5. ASK1 activates two different
subgroups of MAPKK, MKK4 and MKK6, which
in turn activate c-Jun N-terminal kinase (JNK)
and p38 MAP kinase, respectively.
ASK1/MAPKKK5 is activated by TNFR and Fas
through the interaction with members of the
TRAF family and Fas-associated protein Daxx.
Overexpression of ASK1 induces apoptotic cell
death, and a catalytically inactive form of ASK1
inhibit TNF-a-induced apoptosis. ASK1 is
expressed in variety of human and mouse
tissues.
Specificity:
This antibody detects ASK1.
Species Reactivity:
Human, other species not tested.
Host: Rabbit
Isotype: IgG
Immunogen:
Synthetic peptide corresponding to amino acids
1356 to 1375 of human ASK1 protein. Note:
immunogen sequence differs in mouse by last
two amino acids.
Format:
Provided at 0.5 mg/ml in PBS solution
containing 0.02% sodium azide.
Storage and Stability:
Store at -20°C. Aliquot to avoid freeze/thaw
cycles.
Applications and Suggested Dilutions:

Western blot: Use 0.5-1 μg/ml. Detects a 155
kDa band in SW1353 whole cell lysate.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product