Size??100ug
Cat code??200022
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??43kDa
Sensitivity??This antibody detects endogenous levels of Bmi1 and does not cross-react with related proteins.
Immunogen??Purified recombinant human Bmi1 protein fragments expressed in E.coli.
UniProt Number??P35226
GeneBank ID??NM_005180
Subcellular location??Nucleus. Cytoplasm
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername??B lymphoma Mo MLV insertion region (mouse);B lymphoma Mo MLV insertion region 1 homolog;Bmi 1;BMI1;BMI1 polycomb ring finger oncogene;BMI1_HUMAN;Flvi 2/bmi 1;FLVI2/BMI1;MGC12685;Murine leukemia viral (bmi 1) oncogene homolog;Oncogene BMI 1;PCGF 4;PCGF4;Polycomb complex protein BMI 1;Polycomb complex protein BMI-1;Polycomb group protein Bmi1;Polycomb group ring finger 4;Polycomb group RING finger protein 4;RING finger protein 51;RNF 51;RNF51.
Gene_symbol??PCGF4; RNF51; FLVI2/BMI1
Summary??null
UniPort summary  Function??Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A ′Lys-119′, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Application Images :??
Western blot detection of Bmi1 antibody in Hela,A549 and 293T cell lysates using Bmi1 antibody (1:1000 diluted).Predicted band size:43KDa.Observed band size:43KDa.