STAT5A polyclonal antibody
產(chǎn)品名稱： STAT5A polyclonal antibody
英文名稱： STAT5A polyclonal antibody
產(chǎn)品編號： PAB12786
產(chǎn)品價格： null
產(chǎn)品產(chǎn)地： 臺灣
品牌商標(biāo)： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯(lián)系人 :
- 地址 : 臺灣臺北市內(nèi)湖區(qū)洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區(qū)域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of STAT5A.
- Immunogen:
- A synthetic peptide corresponding to residues surrounding Y694 (DGYVK) of human, mouse and rat STAT5A.
- Host:
- Rabbit
- Reactivity:
- Bovine, Dog, Human, Mouse, Rat
- Specificity:
- This antibody recognizes STAT5A without a phosphorylated sites of Tyr694. It does not cross-react with phosphospecific peptide.
- Form:
- Liquid
- Storage Buffer:
- In TBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells.
Lin JX, Mietz J, Modi WS, John S, Leonard WJ.J Biol Chem. 1996 May 3;271(18):10738-44.
- Applications
- Western Blot (Tissue lysate)
- The tissue lysate derived from mouse liver was immunoblotted by STAT5A polyclonal antibody (Cat # PAB12786) at 1 : 500.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical staining of human breast carcinoma tissue stained with STAT5A polyclonal antibody (Cat # PAB12786) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
- Entrez GeneID:
- 6776
- Gene Name:
- STAT5A
- Gene Alias:
- MGF,STAT5
- Gene Description:
- signal transducer and activator of transcription 5A
- Omim ID:
- 601511
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq
- Other Designations:
- -
