抗體,CD90抗體
產(chǎn)品名稱(chēng): 抗體,CD90抗體
英文名稱(chēng): 電詢(xún)
產(chǎn)品編號(hào): YM-S9648-X
產(chǎn)品價(jià)格: null
產(chǎn)品產(chǎn)地: 上海
品牌商標(biāo): 上海遠(yuǎn)慕
更新時(shí)間: null
使用范圍: 科研實(shí)驗(yàn)
- 聯(lián)系人 :
- 地址 : 上海浦東新區(qū)川沙經(jīng)濟(jì)園區(qū)
- 郵編 : 201200
- 所在區(qū)域 : 上海
- 電話 : 133****2040 點(diǎn)擊查看
- 傳真 : 點(diǎn)擊查看
- 郵箱 : m13310162040@163.com
"抗體,CD90抗體
產(chǎn)品貨號(hào):YM-S9648-X
規(guī) 格:0.1ml/0.2ml
濃 度:1mg/1ml.
抗體來(lái)源:Rabbit / mouse
克隆類(lèi)型:兔來(lái)源為polyclonal,鼠來(lái)源為monoclonal
產(chǎn)品類(lèi)型:一抗 單抗
性 狀:凍干粉或液體
亞 型:IgG
純化方法:affinity purified by Protein A
儲(chǔ) 存 液:0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
抗體,CD90抗體Rules:
(1) the initial reaction to produce antibodies to antigens: when thefirst to enter the body, need to produce antibodies after certainincubation period can, and antibody production quantity are not many, inthe body to maintain the time is short.
(2) once again: when the same antigen antibody reaction to producesecond times into the body, at the beginning, as part of the originalantibody binding and once again into the antigen antibody, can make theoriginal amount reduced slightly. Subsequently, rapid increase antibodytiter, several times more than the initial reaction can be generated toa few times, retained in the body of a lot of time.
(3) recall response to produce antibodies: antibodies produced byantigen stimulation of the body, after a certain period of time candisappear gradually. If the re exposure to antigen, the antibody hasfaded fast rise. As the body with the same antigen again first, thenknown as the specific recall responses; and if the initial reactions ofdifferent, it is known as a nonspecific recall response. Increasednonspecific memories to elicit antibody responses are temporary, shorttime soon fall.
抗體產(chǎn)品WB實(shí)驗(yàn)中的對(duì)照系統(tǒng):
Western blotting實(shí)驗(yàn)原理:免疫印跡(immunoblotting)又稱(chēng)蛋白質(zhì)印跡(Western blotting),它是根據(jù)抗原抗體的特異性結(jié)合檢測(cè)復(fù)雜樣品中的某種蛋白的方法。該法是在凝膠電泳和固相免疫測(cè)定技術(shù)基礎(chǔ)上發(fā)展起來(lái)的一種新的免疫生 化技術(shù)。由于免疫印跡具有SDS-PAGE的高分辨力和固相免疫測(cè)定的高特異性和敏感性,現(xiàn)已成為蛋白分析的一種常規(guī)技術(shù)。免疫印跡常用于鑒定某種蛋白, 并能對(duì)蛋白進(jìn)行定性和半定量分析。
WB對(duì)照系統(tǒng):陽(yáng)性對(duì)照:最好有標(biāo)準(zhǔn)品,或陽(yáng)性血清、陽(yáng)性上清。
陰性對(duì)照:測(cè)血時(shí)用相應(yīng)小鼠未免疫血清,測(cè)培養(yǎng)上清,用無(wú)克隆培養(yǎng)上清。空白對(duì)照:不加一抗,用204%BSA代替。無(wú)關(guān)對(duì)照(替代對(duì)照):用無(wú)關(guān)項(xiàng)目一抗,或無(wú)關(guān)項(xiàng)目的抗體。
抗體,CD90抗體相關(guān)產(chǎn)品:
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