Product Highlight

• Apolipoprotein A1 is the major protein component of serum high-density lipoprotein (HDL).

Product Description

Apolipoprotein A1 is the major protein component of serum high-density lipoprotein (HDL). It is produced in the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver, which is thought to inhibit atherogenesis. The plasma concentration of Apolipoprotein A1 is one of the best indicators of susceptibility to cardiovascular disease.

NewEast Biosciences Human Apolipoprotein A1 ELISA Kit is a simple and fast sandwich enzyme immunoassay (EIA) for in vitro quantitative detection of human Apolipoprotein A1 proteins in urine, saliva, milk and cell culture samples. Each kit provides sufficient quantities to perform 96 assays. It works for Apolipoprotein A1 of primates.

Assay Principle

With the NewEast Biosciences Human Apolipoprotein A1 Sandwich ELISA Assay system, mouse monoclonal antibodies generated against human Apolipoprotein A1, are pre-coated onto a 96-well plate and are used to capture human Apolipoprotein A1 from a sample. Captured human Apolipoprotein A1 is detected using human Apolipoprotein A specific rabbit polyclonal antibodies (biotin labeled) and streptavidin-conjugated poly-HRP. After addition of the substrate solutions, the amount of human Apolipoprotein A1 is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and human Apolipoprotein A1 concentration: i.e., the higher the OD the higher the human Apolipoprotein A concentration in the sample.