基本信息

產品名稱Recombinant Human EGF Protein英文名稱Recombinant Human EGF Protein規格或純度GMP, ≥95% (SDS-PAGE), Active, Pichia pastoris, No tag, 971-1023aa生物活性Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <1 ng/mL.內毒素水平≤ 1.0 EU/mg表達系統Pichia pastoris種屬HumanAmino Acids971-1023aa序列NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR蛋白標簽No tag蛋白長度Full length proteinCarrier FreeYesAnimal FreeYesAccession#P01133來源Recombinant預測分子量6.5kDSDS-PAGE7.1 kDa, under reducing conditions.

相關屬性

特點與好處Application: Cell culture, FuncS, HPLC, SDS-PAGE物理外觀Lyophilized復溶建議用無菌水重溶rhEGF凍干粉至100-200μg/ml，可用其他溶劑進行進一步稀釋。溶解后的rhEGF 4℃可保存2-7天，盡快用完。若短期不用，請置于-20℃保存。 開啟后，請盡快使用，避免污染。儲存溫度-20°C儲存運輸條件超低溫冰袋運輸穩定性與儲存Upon delivery aliquot. Store at -20°C . Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.CAS編號和信息62253-63-8

常規信息

基因名EGF基因 ID1950細胞定位Membrane.序列相似性Contains 9 EGF-like domains. Contains 9 LDL-receptor class B repeats.涉及疾病Hypomagnesemia 4英文別名Recombinant Human Epidermal Growth Factor；rhEGF；beta-urogastrone；EGF；epidermal growth factor (beta-urogastrone)；epidermal growth factor；hEGF；HOMG4； pro-epidermal growth factor；URG；Urogastrone別名重組人表皮生長因子；Recombinant Human Epidermal Growth Factor； rhEGF；beta-urogastrone；EGF；epidermal growth factor (beta-urogastrone)；epidermal growth factor； hEGF； HOMG4; pro-epidermal growth factor；URG；Urogastrone

一般描述

pH值：6.5-7.5

人表皮生長因子（EGF）分子量為6.5kD，含有53個氨基酸，分子內有3個二硫鍵。EGF與位于細胞表面的特異受體EGFR 結合后，引起細胞內一系列的生化反應：提高細胞內的鈣離子濃度水平，促進糖酵解的進行，增加蛋白質的合成，并且可以增強EGFR這一類特定基因的表達來促進DNA 的合成和細胞的增殖。

Purity

≥95% SDS-PAGE

Additional sequence information

Mature chain

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Post-translational

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

pH value: 6.5-7.5

Human epidermal cell growth factor, Mannitol, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Water

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Protein Bioactivity: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-Protein Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <1ng/mL.

SDS-PAGE: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-SDS-PAGE

5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 7.1 kDa.

安全信息

信號詞Warning象形圖 ghs07危險聲明H315,H319,H335預防措施聲明P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319WGK Germany3