羥類固醇脫氫酶17β3抗體-抗體-抗體-生物在線
上海雅吉生物科技有限公司
羥類固醇脫氫酶17β3抗體

羥類固醇脫氫酶17β3抗體

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產品名稱： 羥類固醇脫氫酶17β3抗體

英文名稱： HSD17B3

產品編號： 3905

產品價格： null

產品產地： 上海

品牌商標： 雅吉

更新時間： null

使用范圍： WB ELISA IHC-P IHC-F Flow-Cyt IF

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中文名稱 羥類固醇脫氫酶17β3抗體
別????名 17 beta HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; EDH17B3; Estradiol 17 beta dehydrogenase; Estradiol 17 beta dehydrogenase 3; Hydroxysteroid (17 beta) dehydrogenase 3; Testicular 17 beta hydroxysteroid dehydrogenase; Testosterone 17 beta dehydrogenase 3; DHB3_HUMAN.??

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研究領域 腫瘤??細胞生物??免疫學??發育生物學??轉錄調節因子??激酶和磷酸酶??
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,?Mouse,?Rat,? (predicted: Pig,?Cow,?Horse,?Rabbit,?)
產品應用 WB=1:500-2000?ELISA=1:500-1000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=1ug/test?IF=1:100-500?（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分?子?量 34kDa
細胞定位 細胞漿?
性????狀 Liquid
濃????度 1mg/ml
免?疫?原 KLH conjugated synthetic peptide derived from human HSD17B3:101-200/310?
亞????型 IgG
純化方法 affinity purified by Protein A
儲?存?液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 HSD17B3 is involved in the reduction of androstenedione to testosterone. It is expressed predominantly in the testis. Deficiency in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH).

Function:
Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH.

Tissue Specificity:
Testis.

DISEASE:
Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH) [MIM:264300]. These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place.

Similarity:
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

SWISS:
P37058

Gene ID:
3293

Database links:

Entrez Gene: 3293?Human

Omim: 605573?Human

SwissProt: P37058?Human

Unigene: 477?Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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產品圖片 Sample:
Brain (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti-HSD17B3 (bs-3905R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSD17B3 Polyclonal Antibody, Unconjugated(bs-3905R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSD17B3) Polyclonal Antibody, Unconjugated (bs-3905R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-HSD17B3 antibody (bs-3905R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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