Size??100ul
Cat code??200959
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Clone Number??3E7-B5-F2
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??32kDa
Sensitivity??This antibody detects endogenous levels of RPA2 and does not cross-react with related proteins.
Immunogen??Purified recombinant human RPA2 protein fragments expressed in E.coli.
UniProt Number??P15927
GeneBank ID??NM_002946
Subcellular location??Nucleus.Nucleus ? PML body.
Formulation??Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername??60S acidic ribosomal protein P1;AA409079;AI325195;AU020965;HSSB;ik:tdsubc_2g1;M(2)21C;MGC137236;OTTHUMP00000004008;p32;p34;RCJMB04_6d17 replication protein A2, 32kDa;REPA 2;REPA1;REPA2;Replication factor A protein 2;Replication protein A 32 kDa subunit;Replication protein A 32kDa subunit;Replication protein A 34 kDa subunit;Replication protein A;replication protein A1 (70kD);Replication Protein A2 (32kDa);Replication protein A2 32kD;Replication protein A2 32kDa;Replication protein A2;Replication protein A2, 32kDa;RF-A protein 2;Rf-A2;RFA;RFA2_HUMAN;RP A;RP-A p32;RP-A p34;RP21C;RPA 2;RPA 32;RPA;RPA2;RPA32;RPA34;RPA70;RpLP1;RpP2;xx:tdsubc_2g1;zgc:109822.
Gene_symbol??REPA2;RPA32.
Summary??
UniPort summary  Function??Required for DNA recombination,repair and replication.The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Application Images :??
Western blot detection of RPA2 antibody in MCF7,Hela and HUVEC cell lysates using RPA2 antibody (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time：20s.