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<p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">產(chǎn)品簡(jiǎn)介</span></strong></p> <p>人胚腎細(xì)胞株293插入SV40 T-抗原基因后產(chǎn)生的高轉(zhuǎn)染效率的衍生株稱為293T。該細(xì)胞最初的名字293tsA1609neo，攜帶SV40復(fù)制序列，被廣泛用于逆轉(zhuǎn)錄病毒生產(chǎn)、基因表達(dá)和蛋白表達(dá)。</p> <p class="MsoNormal" style="font-size: medium;" align="left"><strong><span style="font-size: 14pt; color: #2e74b5;">使用說(shuō)明</span></strong></p> <table> <tbody> <tr> <td width="123"> <p>細(xì)胞名稱</p> </td> <td width="510"> <p>293T，HEK-293T，人胚腎細(xì)胞</p> </td> </tr> <tr> <td width="123"> <p>別稱</p> </td> <td width="510"> <p>Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo</p> </td> </tr> <tr> <td width="123"> <p>種屬來(lái)源</p> </td> <td width="510"> <p><em>Homo sapiens</em>, human</p> </td> </tr> <tr> <td width="123"> <p>組織來(lái)源</p> </td> <td width="510"> <p>kidney; Embryo</p> </td> </tr> <tr> <td width="123"> <p>細(xì)胞形態(tài)</p> </td> <td width="510"> <p>上皮細(xì)胞樣</p> </td> </tr> <tr> <td width="123"> <p>生長(zhǎng)特性</p> </td> <td width="510"> <p>貼壁生長(zhǎng)</p> </td> </tr> <tr> <td width="123"> <p>生物安全等級(jí)</p> </td> <td width="510"> <p>1級(jí)</p> </td> </tr> <tr> <td width="123"> <p>傳代比例</p> </td> <td width="510"> <p>1:3-1:5</p> </td> </tr> <tr> <td width="123"> <p>換液頻率</p> </td> <td width="510"> <p>每2-3天換液一次</p> </td> </tr> <tr> <td width="123"> <p>生長(zhǎng)培養(yǎng)基</p> </td> <td width="510"> <p>10% FBS、高糖DMEM、1% Penicillin-Streptomycin</p> </td> </tr> <tr> <td width="123"> <p>培養(yǎng)條件</p> </td> <td width="510"> <p>氣相：95% 空氣、5% CO₂；溫度：37<span style="color: black;">℃</span></p> </td> </tr> <tr> <td width="123"> <p>凍存條件</p> </td> <td width="510"> <p>90% FBS + 10% DMSO；液氮</p> </td> </tr> <tr> <td width="123"> <p>支原體檢測(cè)</p> </td> <td width="510"> <p>陰性</p> </td> </tr> <tr> <td width="123"> <p>STR鑒定結(jié)果</p> </td> <td width="510"> <p>D5S818: 8,9</p> <p>D13S317: 12,14</p> <p>D7S820: 11</p> <p>D16S539: 9,13</p> <p>vWA: 16,19</p> <p>THO1: 7,9.3</p> <p>Amelogenin: X</p> <p>TPOX: 11</p> <p>CSF1PO: 11,12</p> </td> </tr> </tbody> </table> <p><strong><span style="color: #2e74b5;">相關(guān)產(chǎn)品</span></strong></p> <table width="643"> <tbody> <tr> <td width="161"> <p><span style="color: black;">產(chǎn)品編號(hào)</span></p> </td> <td width="321"> <p><span style="color: black;">產(chǎn)品名稱</span></p> </td> <td width="161"> <p><span style="color: black;">產(chǎn)品規(guī)格</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03011/JY03012</span></p> </td> <td width="321"> <p>慢病毒濃縮試劑盒</p> </td> <td width="161"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03013/JY03014</span></p> </td> <td width="321"> <p>PEG-8000慢病毒濃縮液</p> </td> <td width="161"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03061/JY03062</span></p> </td> <td width="321"> <p>全套第二代慢病毒包裝質(zhì)粒（三質(zhì)粒系統(tǒng)）</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03063/JY03064</span></p> </td> <td width="321"> <p>全套第三代慢病毒包裝質(zhì)粒（四質(zhì)粒系統(tǒng)）</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03027/JY03028</span></p> </td> <td width="321"> <p>慢病毒包裝質(zhì)粒 pMD2.G</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03029/JY03030</span></p> </td> <td width="321"> <p>慢病毒包裝質(zhì)粒 psPAX2</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03031/JY03032</span></p> </td> <td width="321"> <p>慢病毒包裝質(zhì)粒 pCMV-VSV-G</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03033/JY03034</span></p> </td> <td width="321"> <p>慢病毒包裝質(zhì)粒 pCMV-dR8.91</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03035/JY03036</span></p> </td> <td width="321"> <p>慢病毒包裝質(zhì)粒 pMDLg/pRRE</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03037/JY03038</span></p> </td> <td width="321"> <p>慢病毒包裝質(zhì)粒 pRSV-Rev</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10011</span></p> </td> <td width="321"> <p>pLKO.1 puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10012</span></p> </td> <td width="321"> <p>pLKO.1-EGFP-Puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10013</span></p> </td> <td width="321"> <p>pLVX-Puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10014</span></p> </td> <td width="321"> <p>pLenti-puro</p> </td> <td width="161"> <p><span style="color: black;">1 &mu;g/100 &mu;g</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03041</span></p> </td> <td width="321"> <p>293T，HEK-293T，人胚腎細(xì)胞</p> </td> <td width="161"> <p><span style="color: black;">&gt; 1&times;10? cells</span></p> </td> </tr> </tbody> </table>