Size??100ug
Cat code??200056
Species Cross-reactivity??H,M
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??90kDa
Sensitivity??This antibody detects endogenous levels of UHRF1 and does not cross-react with related proteins
Immunogen??Purified recombinant human UHRF1 protein fragments expressed in E.coil.
UniProt Number??Q96T88
GeneBank ID??NM_013282.3
Subcellular location??Nucleus
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??Np95; hNP95; ICBP90; RNF106; FLJ21925; MGC138707
Gene_symbol??Np95; hNP95; ICBP90; RNF106
Summary??This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
UniPort summary  Function??Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5′-CCAAT-3′ box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
Application Images :??
Western blot detection of UHRF1 antibody in Mouse heart,293T,HelaNE&Hela cell lysates using UHRF1 antibody (1:1000 diluted).
Predicted band size: 90KDa
Observed band size: 90KDa
Immunocytochemistry of HeLa cells using anti- UHRF1 (N-terminus) antibody diluted 1:200.