重組SARS-CoV-2 S蛋白RBD（Fc Tag），GMP級 

英文名稱：Recombinant SARS-CoV-2 Spike Protein-RBD(Fc Tag)

產品貨號：GMP-TL762

產品描述（Description）

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

分子別名（Synonym）

2019-nCoV RBD Protein; 2019-nCoV S1 Protein; 2019-nCoV Spike RBD Protein

表達宿主（Expression host）

CHO細胞

蛋白序列（Protein Sequence）

DNA序列編碼重組SARS-CoV-2 (2019-nCoV)RBD蛋白表達帶有Fc標簽在C末端

分子量（Molecular Weight）

重組SARS-CoV-2 (2019-nCoV) RBD蛋白包含470個氨基酸，預測的理論分子量為52.9kD

純度（Purity）

≥95%采用 SDS-PAGE 凝膠分析和 SEC-HPLC 分析

內毒素（Endotoxin）

≤0.01EU/ug（凝膠法）

提示（Tips）

本蛋白帶有人Fc標簽，應用中會與人源抗體的二抗發生反應，因此請特別注意

提純方法（Purification Method）

層析純化

穩定性（Stability）

1、凍干的樣本可在4℃保存24個月，溶解后的液體可于-20℃保存6-12個月，并且避免反復凍融。

2、液體樣本在-20～-80℃保存12個月，融化后需要進行分裝保存于-20℃保存6-12個月，避免反復凍融。

 ：For research and manufacturing use.