?這種產品利用的是RNA干擾的方法，所謂RNA干擾是指通過雙鏈RNA分子進入細胞可以選擇性地沉默相應基因的表達。針對每個蛋白，我們提供兩個一樣有效的siRNA（siRNA I 和 II）。另外，熒光標記的非目標fluorescein-labeled non-targeted siRNA control #6201 可用作對照監控轉染效率，而非偶聯的對照unconjugated control siRNA #6568 可以用來控制特異性。現在有針對人和小鼠目標蛋白的SignalSilence? siRNA。 ?
?l @??acing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; font-family: Verdana, Helvetica, Arial, sans-serif; line-height: 22.1875px;">?可用作對照監控轉染效率，而非偶聯的對照unconjugated control siRNA #6568?可以用來控制特異性?，F在有針對人和小鼠目標蛋白的SignalSilence^??siRNA。 ?

RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. SignalSilence? Control siRNA (Fluorescein Conjugate) from Cell Signaling Technology (CST) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. In addition, this siRNA is fluorescein-conjugated to allow the researcher to assess transfection efficiency by fluorescence microscopy.

干擾是一種通過向細胞中輸入雙鏈RNA分子，選擇性沉默基因表達的方法。一種特定的小干擾RNA的引入可以通過降解RNA來阻止目的蛋白的翻譯。SignalSilence ? Control siRNA （熒光標記）是一種小干擾RNA（siRNA），不能導致細胞信號的特定降解，而用來作為靶siRNA轉染的陰性對照。另外，這種siRNA帶熒光標記，可以使實驗者通過熒光顯微鏡評估轉染效率。

Fluorescent Detection

Fluorescent detection of SignalSilence? Control siRNA (Fluorescein Conjugate) in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. TransIT-TKO? Transfection Reagent from Mirus was used (Mirus catalog #MIR2114).熒光檢測用SignalSilence ? Control siRNA (熒光標記) 轉染24小時后的活HeLa細胞，轉染效率近100%。 .所用轉染試劑為Mirus公司的TransIT-TKO ? Transfection Reagent (Mirus catalog #MIR2114)。

Western Blotting

Western blot analysis of extracts from HeLa cells, either untransfected or transfected with NF-κB p65 siRNA #6261 or SignalSilence? Control siRNA (Fluorescein Conjugate), using NF-κB p65 Antibody #3034.Western blot分析HeLa細胞提取物。未轉染組和轉染組用NF-κB p65 siRNA #6261 或 SignalSilence ? Control siRNA (熒光表記)，所用抗體為 NF-κB p65 Antibody #3034.

Directions for Use

CST recommends transfection with 100 nM SignalSilence? Control siRNA (Fluorescein Conjugate) 24 to 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

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建議裂解細胞前用100nM Control siRNA II 轉染48-72個小時。請參考轉染試劑生產商提供的轉染步驟。如有問題，請聯系CST公司。

6202 SignalSilence? ILK1 siRNA I

6211 SignalSilence? Akt siRNA I

6231 SignalSilence? p53 siRNA I

6241 SignalSilence? Chk1 siRNA I

6251 SignalSilence? PTEN siRNA I

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6261 SignalSilence? NF-κB p65 siRNA I