HIPREP 16/60 SEPHACRYL S-100 H
產品名稱： HIPREP 16/60 SEPHACRYL S-100 H
英文名稱： HIPREP 16/60 SEPHACRYL S-100 H
產品編號： 17-1165-01
產品價格： 0
產品產地： 美國
品牌商標： GE
更新時間： null
使用范圍： null
| 17-1165-01 | HIPREP 16/60 SEPHACRYL S-100 H | 1 × 120 ml |
| 17-1166-01 | HIPREP 16/60 SEPHACRYL S-200 H | 1 × 120 ml |
| 17-1167-01 | HIPREP 16/60 SEPHACRYL S-300 H | 1 × 120 ml |
| 17-1194-01 | HIPREP 26/60 SEPHACRYL S-100 H | 1 × 320 ml |
| 17-1195-01 | HIPREP 26/60 SEPHACRYL S-200 H | 1 × 320 ml |
| 17-1196-01 | HIPREP 26/60 SEPHACRYL S-300 H | 1 × 320 ml |
HiPrep? Sephacryl? HR Columns and Sephacryl? Lab Packs
Technical Information
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HiPrep? Sephacryl? HR columns, prepacked with Sephacryl? High Resolution media, provide reliable gel filtration separations.
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HiPrep? Sephacryl? HR prepacked gel filtration columns offer excellent separation properties in a convenient, ready-to-use format. By combining the performance of Sephacryl? High Resolution media with a low cost column construction, they are both reliable and cost effective to use with simple equipment or systems.
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Five HiPrep? Sephacryl? HR columns are available prepacked with S-100, S-200, S-300, S-400, or S-500 HR media, respectively. Each column is also available in two sizes: 16/60 for sample volumes of up to 5 ml and 26/60 for sample volumes of up to 13 ml. The range of HiPrep? Sephacryl? HR prepacked columns allows purification of molecules with molecular weights as small as 1 × 103 to macromolecules as large as 2 × 107.
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| TECHNICAL SPECIFICATIONS | |
| Sephacryl? High Resolution | |
| Fractionation range (Mr) -? | ? |
| - Globular proteins? | ? |
| ? S-100 HR ? S-200 HR ? S-300 HR ? S-400 HR ? |
1 × 103 - 1 × 105 5 × 103 - 2.5 × 105 1 × 104 - 1.5 × 106 2 × 104 - 8 × 106? |
| - Dextrans? | ? |
| ? S-200 HR ? S-300 HR ? S-400 HR ? S-500 HR ? S-1000 SF? |
1 × 103 - 8 × 104 2 × 103 - 4 × 105 1 × 104 - 2 × 106 4 × 104 - 2 × 107 5 × 105 - > 108? |
| ? Exclusion limit up to 400 nm for spherical particles.? | |
| - DNA exclusion limit? | ? |
| ? S-200 HR ? S-300 HP ? S-400 HR ? S-500 HR ? S-1000 SF? |
30 bp 118 bp 271 bp 1078 bp 20?000 bp? |
| Matrix? | Spherical allyl dextran and N, N’-methylenebisacrylamide? |
| Chemical stability? | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine-HCl, 1% SDS, 2 M NaCl, 24% ethanol, 30% propanol, 30% acetonitrile, 0.5 M NaOH (for cleaning)? |
| pH stability? | 3-11 (long term and working) 2-13 (short term)? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
| Physical stability ? Autoclavability ? Antimicrobial agent? |
Negligible volume variation due to changes in pH or ionic strength ? At 121°C, pH 7 for 30 minutes (gel only) ? 20% ethanol? |
| ? Autoclavability? | ? At 121°C, pH 7 for 30 minutes (gel only)? |
| ? Autoclavability? | ? At 121°C, pH 7 for 30 minutes (gel only)? |
| ? Antimicrobial agent? | ? 20% ethanol? |
| ? Autoclavability? | ? At 121°C, pH 7 for 30 minutes (gel only)? |
| ? Antimicrobial agent? | ? 20% ethanol? |
| ? Autoclavability? | ? At 121°C, pH 7 for 30 minutes (gel only)? |
| ? Antimicrobial agent? | ? 20% ethanol? |
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Selectivity of the five different prepacked HiPrep Sephacryl? HR columns.
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| TECHNICAL SPECIFICATIONS | |
| Prepacked HiPrep? Sephacryl HR Columns | |
| Bed dimensions? | ? |
| ? HiPrep? 16/60 ? HiPrep? 26/60? |
16 × 600 mm 26 × 600 mm? |
| Bed volume? | ? |
| ? HiPrep? 16/60 ? HiPrep? 26/60? |
120 ml 320 ml? |
| Sample volume? | ? |
| ? HiPrep? 16/60 ? HiPrep? 26/60? |
< 5 ml < 13 ml? |
| Rec. linear flow rate? | 5-15 cm/h? |
| Max. linear flow rate? | 30 cm/h? |
| (H2O at 25°C)? | ? |
| Max. pressure over the?packed bed during operation? | 1.5 bar (22 psi, 0.15 MPa)? |
| Column hardware pressure limit? | 5 bar (73 psi, 0.5 MPa)? |
| Theoretical plates? | 5000 m-1? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
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