Size??100ul
Cat code??220013
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Clone Number??6H7-B3-C8
Antibody type??Monoclonal antibody
Purified method??
Isotype??IgG1
Molecular Weight??65kDa
Sensitivity??The antibody detects endogenous level of total RELA protein.
Immunogen??Purified recombinant fragment of human NF-κB p65 expressed in E. Coli.
UniProt Number??Q04206
GeneBank ID??NM_021975
Subcellular location??Nucleus. Cytoplasm. Note: Colocalized with DDX1 in the nucleus upon TNF-alpha induction . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation.
Formulation??Ascitic fluid containing 0.03% sodium azide.
Altername??Avian reticuloendotheliosis viral (v rel) oncogene homolog A ; MGC131774 ; NF kappa B p65delta3 ; NFKB 3 ; NFKB3 ; NFKB3 ; Nuclear Factor NF Kappa B p65 Subunit ; Nuclear factor NF-kappa-B p65 subunit ; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 ; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells ; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 ; OTTHUMP00000233473 ; OTTHUMP00000233474 ; OTTHUMP00000233475 ; OTTHUMP00000233476 ; OTTHUMP00000233900 ; p65 ; p65 NF kappaB ; p65 NFkB ; RELA ; TF65_HUMAN ; Transcription Factor p65 ; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) ; V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A ; v rel reticuloendotheliosis viral oncogene homolog A (avian) ; V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 ; v-rel reticuloendotheliosis viral oncogene homolog A ;
Gene_symbol??p65; NFKB3
Summary??NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniPort summary  Function??NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
Application Images :??
Western blot detection of RELA antibody in 3T3, K562 and Hela cell lysates using RELA antibody(dilution 1:500).Predicted band size:65KDa.Observed band size:65KDa.