IDH2(R172K) 小鼠單抗
產品名稱： IDH2(R172K) 小鼠單抗
英文名稱： Anti-IDH2(R172K) Mouse Monoclonal Antibody
產品編號： 26163
產品價格： null
產品產地： 美國
品牌商標： Neweast Biosciences
更新時間： null
使用范圍： ELISA, WB, IHC,IF
武漢費斯德生物科技有限公司
- 聯系人 :
- 地址 : 武漢東湖新技術開發區高新大道666號武漢國家生物產業基地項目（生物創新園）B.C.D區研發樓B3-3棟3樓
- 郵編 :
- 所在區域 : 湖北
- 電話 : 181****2833 點擊查看
- 傳真 : 點擊查看
- 郵箱 : nebio0923@163.com
| 貨號: ??26163 |
| 基因符號: ??IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| 描述: ??Anti-IDH2(R172K) Mouse Monoclonal Antibody |
| 背景介紹: ??Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. |
| 免疫原: ??A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin |
| 經過測試的應用: ??ELISA, WB, IHC |
| 推薦稀釋比: ???ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IHC: 1: 10-1: 100 |
| 濃度: ??1 mg/ml |
| 宿主: ??Mouse |
| 形式: ??Liquid |
| 克隆性: ??Monoclonal |
| 亞型:? ?IgG |
| 純度: ??Purified from ascites |
| 防腐劑:?No |
| 成分:???PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 種屬反應性: ??recognizes R172K mutant, but not wild-type IDH2 of vertebrates |
| 儲存條件: ???Store at -20°C. Avoid repeated freezing and thawing |
蛋白質印跡: ?? Western blot analysis of recombinant IDH2(R172K) and wildtype proteins.?Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #26163).?免疫熒光: ??  Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody.?HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163). |