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Fibroblast growth factor-21 (FGF21) belongs to the large FGF family which has at least 23 members. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Recombinant Mouse Fibroblast Growth Factor-21(FGF-21) produced in E.coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rmFGF-21 has a molecular mass of 19.9 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at ExCell Bio.