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產品名稱：鈉鉀ATP酶蛋白a1抗體說明書
產品貨號：YM-H18375-X
濃 度： 1mg/1ml
貯 存： 貯存于-20℃.
亞 型： IgG
性 狀：Lyophilized or Liquid
產品類型： 一抗 多抗體
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
鈉鉀ATP酶蛋白a1抗體說明書產品WB實驗中的對照系統：
he chromogenic reaction （Note: two anti generally have two kinds ofcommonly used marker enzyme, HRP and AP, chromogenic substrate kit andits corresponding to DAB and BCIP/NBT, the general use ofchemiluminescence lysate.） Westernbloting is the first to transfer afterelectrophoresis separation of proteins from the gel onto a NC membrane,there are generally two ways: capillary blotting and electrophoreticblotting. Westernbloting is a common method used to detect the protein,through the immune reaction of antigen antibody can be quantitativelymeasured the relative molecular weight of antibody, and the relativeabundance of known antigen or antibody and the relation between. Atpresent, it is applied in many fields, detection of infectious diseasesand autoimmune diseases, allergies, etc. in clinic, in antibodyproduction field it with ELISA, ICC, IHC, IFA and other immunetechnology has become the main means of engaging a screening ofmonoclonal antibody production.
WB對照系統
陽性對照：最好有標準品，或陽性血清、陽性上清。
陰性對照：測血時用相應小鼠未免疫血清，測培養上清，用無克隆培養上清。
空白對照：不加一抗，用1%BSA代替。
無關對照（替代對照）：用無關項目一抗，或無關項目的抗體。
The principle of immunohistochemical method:
1. Immunofluorescence cytochemistry technology
2. Immune enzyme cytochemistry technology: within the cell or tissue on the corresponding antigen to locate or qualitative research.
3. Immune colloidal gold technique: with colloidal gold mark is A resistance, the combination of the two can resist or other specific immunoglobulin molecules (such as staphylococcus protein A) as A probe to qualitative tissue or cell antigen, positioning and quantitative research.
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