重組人PD-1蛋白(His)（Recombinant Human PD-1 Protein(His)），GMP級

產品貨號：GMP-TL756

產品描述（Description）

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

分子別名（Synonym）

PDCD1、D279、SLEB2

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列編碼人 PD-1（ NCBI Reference Sequence: NP_005009.2）表達帶有 His 標簽在 C 末端

分子量（Molecular Weight）

重組人 PD-1 蛋白包含 148 個氨基酸，預測理論分子量為 21.16kD

純度（Purity）

≥95%采用SDS-PAGE凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 PD-1(His 標簽)0.5ug/mL (100uL/孔) 能夠結合人 PD-L1(Fc 標簽) 的線性范圍是 0.5-10ug/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6~12個月，并且避免反復凍融 

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1. PD-1 Expression on Mycobacterium tuberculosis-Specific CD4 T Cells Is Associated With Bacterial Load in Human Tuberculosis Cheryl L. Day, Deborah A. Abrahams, Rubina Bunjun, Lynnett Stone, Marwou de Kock, Gerhard Walzl, Robert J. Wilkinson, Wendy A. Burgers, Willem A. Hanekom Front Immunol. 2018; 9: 1995. Published online 2018 Aug 31. doi: 10.3389/fimmu.2018.01995

  注意：For research and manufacturing use.