SPECIFICATION?SHEET

Catalog?#:????L1C00802??????????????Lot?#:?????????C080401

Description:?????MAb?to?Cancer?Antigen?CA15-3
????Monoclonal?Antibody?to?Cancer?Antigen?CA15-3?(Breast?Cancer)

Specificity:????Specific?for?the?tandem?repeat?of?the?MUC-1?core.?Recognizes?the?hexapeptide?(TRPAPG).?The?MUC-1?mucin?(Cancer?Antigen?CA15-3)?is?secreted?from?tumor?cells.?The?apo-protein?of?the?MUC-1?mucin?contains?a?transmembrane?domain,?a?cytoplasmic?domain,?and?an?extracellular?carbohydrate?rich?domain.?The?extracellular?domain?is?characterized?by?polymorphism?with?respect?to?the?number?of?a?20?amino?acid?tandem?repeat?(VNTR?polymorphism).

Clone:????ML04H002

Host?Animal:????Mouse.?Hybridization?of?P3X63-Ag8.653????

Isotype:?????IgG1?myeloma?cells?with?spleen?cells?from?Balb/c?mice.
????
Source:?????Mouse?Ascites

Immunogen:????Human?breast?cancer?cell?line?ZR?75-1

Format:????Purified,?Liquid

Purification:??????>98%?pure.??Protein?A?chromatography

Concentration:?????3.0mg/ml?(OD280nm)

Affinity?Constant:??????Not?determined

Buffer:????150mM?NaCl?10mM?Tris??PH7.4

Preservative:?????0.05%NaN3

Applications:?????Capture?or?detection?antibody?in?serological?assays?for?the?research?of?antigen?MUC-1.?Forms?a?suitable?pair?with?ML04H001,?Catalog?#?L1C00801.?Immunohistochemistry?on?frozen?or?paraffin?sections?for?the?detection?of?MUC-1.?Immunochemical?detection?of?MUC-1?protein.?Each?laboratory?should?determine?an?optimum?working?titer?for?use?in?its?particular?application.?Other?applications?have?not?been?tested?but?use?in?such?assays?should?not?necessarily?be?excluded.

Storage:????Short-term?(up?to?2?months)?store?at?<-20°C.?Long?term?(>2?months)?store?at?<-40°C.??Avoid?multiple?freeze/thaw?cycles.