Flow cytometric analysis of BAX expression in HT-1080 cells using BAX antibody (Cat#CAS-2918, 1:2,000). Green, isotype control; red, BAX.

Immunocytochemical staining of HT-1080 cells with BAX antibody (Cat#CAS-2918, 1:1,000) . Nuclei were stained blue with DAPI; BAX was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using BAX antibody (Cat#CAS-2918). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAX antibody (Cat#CAS-2918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#CAS-212, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#CAS-237).