女人与狗锁死了视频大全|霍金死亡过程恐怖视频|无节社团简谱季免费观看|六间房直播大厅|中文字幕欧美另类精品|久久人人玩人妻潮喷内射人人|亚洲国产一区二区三区在线观看

來源
用途
交叉反應性
分子量
Rabbit
WB, IF, IHC, ICC
H, M
70/60 kDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1,000
-
1:50-1:200
1:50-1:200
1:50-1:200
-
-
-
抗體詳細信息如下:
About this Antibody
Name
p73 Rabbit Monoclonal Antibody
Category
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype
IgG
Purification
Affinity purification
About the Immunogen
Immunogen
Recombinant protein
Gene ID
7161(Human); 22062(Mouse)
SwissProt
O15350(Human); Q9JJP2(Mouse)
Synonyms
P73
Category
Cell Cycle
Background
The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents. The p73 isoform p73α inhibits drug-induced apoptosis in small cell lung carcinoma cells, while the p73 isoform p73β promotes it. p73α also prevents Bax activation, mitochondrial dysfunction, caspase activation and is able to reduce apoptosis induced by the BH3-only protein PUMA (p53 upregulated modulator of apoptosis). There is an equilibrium between p73α and p73β, demonstrated by the fact that p73α inhibits the pro-apoptotic effect of p73β.
包裝清單:
產(chǎn)品編號
產(chǎn)品名稱
包裝
AF1876
p73 Rabbit Monoclonal Antibody
50μl
AZ050
Western一抗稀釋液
50ml
-
說明書
1份
保存條件:
p73 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
代表性圖片：
Fig. 1. Western blot analysis of p73 on Hela celllysates using anti-p73 antibody at 1/1,000 dilution.
Fig. 2. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-p73 antibody. Counter stained with hematoxylin.
?