HITRAP NHS-ACTIV HP-色譜柱 -耗材-生物在線
上海基星生物科技有限公司
HITRAP NHS-ACTIV HP

HITRAP NHS-ACTIV HP

商家詢價

產品名稱: HITRAP NHS-ACTIV HP

英文名稱: HITRAP NHS-ACTIV HP

產品編號: 17-0716-01

產品價格: 0

產品產地: USA

品牌商標: 美國GE

更新時間: null

使用范圍: null

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17-0716-01 HITRAP NHS-ACTIV HP, 5 X 1ML
17-0716-30 HiTrap NHS-activated HP, 180 x
17-0717-01 HITRAP NHS-ACTIV HP, 1 X 5 ML
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17-0978-13 HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14 HITRAP BUTYL-S FF, 5 X 5ML
17-1128-01 MABTRAP KIT
17-1151-01 HITRAP SP HP, 5 X 1 ML
17-1152-01 HITRAP SP HP,?5X5 ML
17-1153-01 HITRAP Q HP, 5 X 1 ML
17-1154-01 HITRAP Q HP 5X5 ML
17-1351-01 HITRAP PHENYL HP, 5 X 1ML
17-1353-01 HITRAP PHENYL FF (LS), 5X1ML
17-1355-01 HITRAP PHENYL FF (HS),5 X 1ML
17-1357-01 HITRAP BUTYL FF, 5 X 1 ML
17-1359-01 HITRAP OCTYL FF, 5 X 1 ML
17-1408-01 HITRAP DESALTING, 5X5ML
17-5053-01 HITRAP Q FF, 5 X 1 ML
17-5054-01 HITRAP SP FF, 5 X 1 ML
17-5055-01 HITRAP DEAE FF 5 X 1 ML
17-5056-01 HITRAP CM FF, 5 X 1 ML

HiTrap? NHS-activated HP Columns

  • Prepacked with NHS-activated Sepharose? High Performance for coupling ligand via primary amines at near neutral pH.
  • Simple coupling, carried out at close to neutral pH.
  • Delivered in 100% isopropanol to protect the active groups.

HiTrap? NHS-activated HP Columns

Technical Information


Click here to view this image in high resolution
Lane 1: Desorbed material, reduced
Lane 2: Low Molecular Weight (LMW) calibration kit, reduced
Lane 1: Desorbed material, non-reduced
Lane 2: Low Molecular Weight (LMW) calibration kit, reduced

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The use of NHS-activated Sepharose? High Performance is illustrated by the purification of anti-mouse Fc-IgG from sheep antiserum after coupling mouse IgG to HiTrap? NHS-activated. The contents of the different fractions were analysed by SDS-PAGE using PhastSystem? and Goomassie staining.
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TECHNICAL SPECIFICATIONS
Group to be coupled? -NH2?
Coupling conditions? pH 6.5-9 at 4°C to 25°C?
Matrix? Highly cross-linked agarose, 6%?
Substitution? 10 μmol NHS/ml medium?
Spacer arm? 6-aminocaproic acid, 10-atom?
pH stability*? 3-12?
Average particle size? 34 μm?
Storage? 100% isopropanol?
Storage temperature? 4°C to 8°C?
* Ligand dependent.

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For column specifications, see Prepacked HiTrap? Affinity Columns.

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Click here to view this image in high resolution
Purification of anti-mouse Fc-IgG from sheep antiserum.