• 靶標種屬Rabbit
• 特異性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
• 經測試應用ICC,?IHC-P,?ELISA,?WBmore details
• 偶聯物HRP

性能

• 形式Liquid
• 存放說明Shipped at 4°C. Store at +4°C.
• 存儲溶液Preservative: 0.1% Proclin
  Constituents: 0.2% BSA, PBS
• 濃度1 mg 濃度為 1 mg/ml?
  
• 純度Immunogen affinity purified
• 純化說明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
• 共軛說明Molar enzyme/ antibody protein ratio is 4:1
• 克隆多克隆
• 同種型IgG
• 常規說明Part of the?AbExcel?range.
• 研究領域
  • Immunology
  ?
  • Immunoglobulins
  ?
  • Heavy Chain
  ?
  • IgG

  • Secondary antibodies
  ?
  • anti-Rabbit
  ?
  • IgG
  ?
  • Enzyme
  ?
  • HRP

應用

Goat Anti-Rabbit IgG H&L (HRP) 圖像

• 

  Western blot - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)This image is courtesy of an anonymous Abreview.
  
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time :?1 minute

  This image is courtesy of an anonymous Abreview.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

  IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with?ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97051,?1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is secondary-only at 1/500 dilution.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051)
  All lanes :?Anti-beta Actin antibody - Loading Control (ab8227) at 1 μg/ml
  
  Lanes 1-6 :?HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

  Lysates/proteins at 10 μg per lane.
  
  Secondary
  Lanes 1 - 2 :?Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
  Lanes 3 - 4 :?Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
  Lanes 5 - 6 :?Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution
  
  Developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time :?10 seconds