Recombinant Human GDNF
 
Catalog Number:
AF-450-10
Synonyms:
Glial-Derived Neurotrophic, Factor, ATF-1
Description:
?
GDNF是一種由二硫鍵連接的同源二聚體神經營養因子，結構上與Artemin、Neurturin和Persephin相關。這些蛋白屬于生長因子的半胱氨酸結(cysteine-knot)超家族，具有穩定的二聚體蛋白結構。GDNF通過一個多組份受體系統傳遞信號，該系統由RET(神經酪氨酸激酶)和四個GFR?(?1-?4)受體之一組成。GDNF特異性促進多巴胺攝取，以及中腦神經元的存活和形態分化。用帕金森氏癥小鼠模型證實，GDNF能夠改善動作徐緩、肌強直和姿勢不穩等癥狀。具有功能的小鼠GDNF配體是由二硫鍵連接而成的同源二聚體，含兩條15.kDa的多肽鏈單體。每個單體均有七個保守的半胱氨酸殘基，其中一個(Cys 101)用于形成鏈間二硫鍵橋，而其它幾個參與分子內環，即半胱氨酸結的形成。生產過程中的所有試劑均為非動物源性。
AA Sequence:
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Source:
E.coli
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by the proliferation of rat C6 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
?
?
?
?
Cross Reactivity:
Human
Research Interests:
Angiogenesis/ Cardiovascular
Neurobiology
Stem Cells & Differentiation
TGF-β Superfamily
?
?
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.