重組人TNFRSF14蛋白-細(xì)胞/細(xì)菌培養(yǎng)-試劑-生物在線

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蘇州近岸蛋白質(zhì)科技股份有限公司
重組人TNFRSF14蛋白

重組人TNFRSF14蛋白

商家詢價(jià)

產(chǎn)品名稱: 重組人TNFRSF14蛋白

英文名稱:

產(chǎn)品編號(hào): C418

產(chǎn)品價(jià)格: 0

產(chǎn)品產(chǎn)地: 上海

品牌商標(biāo): novoprotein

更新時(shí)間: null

使用范圍: null

蘇州近岸蛋白質(zhì)科技股份有限公司
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目錄號(hào) 規(guī)格 價(jià)格 說明書下載
C418A 2μg 400元
C418B 10μg 1800元
C418C 50μg 4500元

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TNFRSF14, also known as Herpesvirus entry mediator (HVEM), TR2 (TNF receptor like molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. The extracellular domain of HVEM contains several cysteine rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor associated factor) interaction domain. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus(HSV) envelope glycoprotein D which plays an important role in HSV pathogenesis. Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT competes with HSV glycoprotein D for HVEM.The cytoplasmic region of HVEM was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response


·Description
Recombinant Human TNFRSF14 produced by transiently transfected human cell is the extracellular domain(Accession#Q92956) (Leu39-Val202) of human TNFRSF14 fused with a polyhistidine tag at the C-terminus.


·Quality Control

Biological activity:The ED50 of rHuTNFRSF14 is typically 2.5-10μg/mL in the presence of 1 ng/mL of rhTNF?, as measured by its ability to inhibit TNF? mediated cytotoxicity using L929 mouse fibrosarcoma cells.

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: Predicted N terminal amino acid is Leu39.

Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHuTNFRSF14.

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·Formulation
Sterile and Lyophilized from a 0.2 μm filtered solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized rHuTNFRSF14 in sterile water not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

·Storage
Lyophilized rHuTNFRSF14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuTNFRSF14 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples containing 0.1% BSA are stable up to 3 months below -18°C for future use.