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IL-1α is a non-secreted, proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1a and IL-1b bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1b is a secreted cytokine, IL-1a is predominantly a cell-associated cytokine.
The BioGems Rat IL-1α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat IL-1α with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-1Α. It contains E. coli-expressed recombinant Rat IL-1Α. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-1Α. The kit is analytically validated with ready to use reagents.