人活性拓撲異構酶 全長蛋白質
產品名稱： 人活性拓撲異構酶 全長蛋白質
英文名稱： DNA topoisomerase 1 DNA topoisomerase I NUP98 fusion gene TOP 1 TOP I TOP1 TOP1_HUMAN TOPI Topoisomerase (DNA) I Topoisomerase 1 Topoisomera
產品編號： ab3808
產品價格： 0
產品產地： 英國
品牌商標： abcam
更新時間： null
使用范圍： null
- 聯系人 :
- 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
- 郵編 :
- 所在區域 : 廣東
- 電話 : 133****4454 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 1484332550@qq.com
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- 性質Recombinant
- 來源Baculovirus
- 氨基酸序列
- 種屬Human
- 分子量91 kDa
技術指標
Our?Abpromise guarantee?covers the use of?ab3808?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 生物活性
A unit of topo I can relax 0.2 ug pGEM 5Z DNA in 30 min at 37oC. The enzyme is supplied at a nominal concentration of approximately 2 units/ul.
- 應用
Functional Studies
Western blot
- 形式Liquid
- 補充說明
Quality Control Tests: 1. A test for nuclease contamination is carried out by assaying for the formation of linear KDNA and linear plasmid DNA. Incubation with KDNA or supercoiled pGEM 5Z DNA (4 hrs. at 37oC in the presence of 10 mM MgCl2) is performed. Linear DNA or breakdown products are not generated under these conditions. 2. A check for cross contamination with Topo II is negative. There must be no decatenation of KDNA in topo II reaction conditions. 3. Performance in converting super-coiled DNA to relaxed DNA.
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制備和貯存
- 穩定性和存儲
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常規信息
-
別名
- DNA topoisomerase 1
- DNA topoisomerase I
- NUP98 fusion gene
see all - 功能The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
- 疾病相關Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
- 序列相似性Belongs to the eukaryotic type I topoisomerase family.
- 翻譯后修飾Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
- 細胞定位Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
- Information by UniProt
Active human Topoisomerase I full length protein 圖像
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SDS-PAGE - Active human Topoisomerase I full length protein (ab3808)Coomassie Blue staining of purified human DNA topoisomerase protein. -
pGEM processed by Topoisomerase I