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Product: Anti-Adiponectin Receptor 1 pAb
Cat. No.: PC-103 (100 μL)
Synonyms:
Anti-AdipoR1, anti-CGI-45.
Specificity:
Recognizes human adiponectin receptor 1.
Species Reactivity:
Human. Others not tested.
Source:
Rabbit
Immunogen:
Synthetic peptide corresponding to aa 2 - 36
(S2SHKGSVVAQGNGAPASNREADTVELAELG
PLLEE36) of human adiponectin receptor 1.
Format:
100 μL of neat liquid serum containing 0.02%
sodium azide.
Storage and Stability:
Store at 4°C short term, -20°C long term. Aliquot
to avoid freeze/thaw cycles.
Application and Suggested Dilution:

Western blotting: Use at a 1:1,000 dilution.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Detection of Adiponectin Receptor 1 (human) in
HEK 293T transfected cells by PC-103.
Method: 10 μg per lane of total cell extracts from mock
transfected HEK 293T cells (lanes 1 and 3) or HEK 293T
cells transfected with an expression vector encoding the
Flag-tagged human AdipoR1 (lanes 2 and 4) were run on a
12% SDS-PAGE gel and analyzed by Western blot. Lanes 1
and 2 were probed with an anti-FLAG antibody at a 1:2,000
dilution. Lanes 3 and 4 were probed with PC-103 at a
1:1,000 dilution. After 1 hour of incubation at RT, the
membrane was extensively washed and then incubated with
goat anti-mouse horseradish peroxidase or a goat anti-rabbit
horseradish peroxidase, respectively, for ECL detection.
Note that the upper bands might represent oligomerized
forms of the receptor.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.