HITRAP IMAC HP
產(chǎn)品名稱： HITRAP IMAC HP
英文名稱： HITRAP IMAC HP
產(chǎn)品編號： 17-0920-03
產(chǎn)品價格： 0
產(chǎn)品產(chǎn)地： 美國
品牌商標： GE
更新時間： null
使用范圍： null
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IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns
- For optimizing purification of histidine-tagged* proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Small bead size for high performance purification.
- High binding capacity.
- Prepacked HiTrap columns for convenient purification.
IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns
Technical Information
<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
* For licensing information, see Legal Info.
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| TECHNICAL SPECIFICATIONS | |
| Matrix? | Highly cross-linked agarose, 6%? |
| Average particle size? | 34 μm? |
| Binding capacity?? | At least 40 mg histidine-tagged protein/ml medium when charged with Ni2+? |
| Compatibility? | Stable in all commonly used buffers? |
| Chemical stability*? | For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1M NaOH, 70% acetic acid 30 min tested 30% 2-propanol 1 h tested 2% SDS? |
| pH stability*? | 3-12 (long term), 2-14 (short term)? |
| HiTrap? prepacked columns | |
| Column volume? | 1 ml and 5 ml? |
| Column dimensions? | 0.7 × 2.5 cm (1 ml), 1.6 × 2.5 cm (5 ml)? |
| Recommended flow rate?? | 1 ml/min (1 ml), 5 ml/min (5 ml)? |
| Max. pressure?? | 3 bar (0.3 MPa, 42 psi)? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
| * Medium uncharged with metal ions. ? H2O at room temperature. ? Protein- and metal-ion dependent. ? |
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<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
Purification of GFP-(His)6 from E. coli lysate using HiTrap IMAC HP column charged with different metal ions.
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<sub>6</sub> fractions purified with different metal ions according to the chromatogram above. '))
SDS-PAGE of GFP-(His)6 fractions purified with different metal ions according to the chromatogram above
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=166316