HITRAP IMAC HP-色譜柱 -耗材-生物在線
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HITRAP IMAC HP

HITRAP IMAC HP

商家詢價

產品名稱： HITRAP IMAC HP

英文名稱： HITRAP IMAC HP

產品編號： 17-0920-03

產品價格： 0

產品產地： 美國

品牌商標： GE

更新時間： null

使用范圍： null

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17-0920-03 HITRAP IMAC HP, 5 X 1 ML
17-0920-05 HITRAP IMAC HP, 5 X 5 ML
17-0921-02 HITRAP IMAC FF, 5 X 1ML
17-0921-04 HITRAP IMAC FF, 5 X 5 ML
17-0978-13 HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14 HITRAP BUTYL-S FF, 5 X 5ML
17-1128-01 MABTRAP KIT
17-1151-01 HITRAP SP HP, 5 X 1 ML
17-1152-01 HITRAP SP HP,?5X5 ML
17-1153-01 HITRAP Q HP, 5 X 1 ML
17-1154-01 HITRAP Q HP 5X5 ML
17-1351-01 HITRAP PHENYL HP, 5 X 1ML
17-1353-01 HITRAP PHENYL FF (LS), 5X1ML
17-1355-01 HITRAP PHENYL FF (HS),5 X 1ML
17-1357-01 HITRAP BUTYL FF, 5 X 1 ML
17-1359-01 HITRAP OCTYL FF, 5 X 1 ML
17-1408-01 HITRAP DESALTING, 5X5ML
17-5053-01 HITRAP Q FF, 5 X 1 ML
17-5054-01 HITRAP SP FF, 5 X 1 ML
17-5055-01 HITRAP DEAE FF 5 X 1 ML
17-5056-01 HITRAP CM FF, 5 X 1 ML

IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns

  • For optimizing purification of histidine-tagged* proteins when Ni2+ is not the best choice of metal ion.
  • Conveniently charge with your metal of choice.
  • Small bead size for high performance purification.
  • High binding capacity.
  • Prepacked HiTrap columns for convenient purification.

IMAC Sepharose High Performance Media/HiTrap IMAC HP Columns

Technical Information


Click here to view this image in high resolution
* For licensing information, see Legal Info.

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TECHNICAL SPECIFICATIONS
Matrix? Highly cross-linked agarose, 6%?
Average particle size? 34 μm?
Binding capacity?? At least 40 mg histidine-tagged protein/ml medium when charged with Ni2+?
Compatibility? Stable in all commonly used buffers?
Chemical stability*? For one week at 40°C: 0.01 M HCl, 0.1 M NaOH
For 12 h: 1M NaOH, 70% acetic acid
30 min tested 30% 2-propanol
1 h tested 2% SDS?
pH stability*? 3-12 (long term), 2-14 (short term)?
HiTrap? prepacked columns
Column volume? 1 ml and 5 ml?
Column dimensions? 0.7 × 2.5 cm (1 ml), 1.6 × 2.5 cm (5 ml)?
Recommended flow rate?? 1 ml/min (1 ml), 5 ml/min (5 ml)?
Max. pressure?? 3 bar (0.3 MPa, 42 psi)?
Storage? 20% ethanol?
Storage temperature? 4°C to 30°C?
* Medium uncharged with metal ions.
? H2O at room temperature.
? Protein- and metal-ion dependent.

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Click here to view this image in high resolution
Purification of GFP-(His)6 from E. coli lysate using HiTrap IMAC HP column charged with different metal ions.

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SDS-PAGE of GFP-(His)6 fractions purified with different metal ions according to the chromatogram above

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