Product Highlight

• Configuration-specific monoclonal antibody that recognizes Arl1-GTP, but not Arl1-GDP
• The only available Arl1 activation assay kit directly measures the level of Arl1-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Arl1 in cells and in tissues by immunohistochemistry

Product Description

Arf-like protein 1 (Arl1) is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases, and with highly conserved orthologs throughout eukaryotes. Arl1 is essential for early embryonic development inDrosophila?and in?Caenorhabditis elegans. Arl1 is most similar in primary sequence, cellular location, and function (regulation of membrane traffic) to Arf1-6 and even shares several common binding partners. In addition to its function in membrane traffic at the Golgi/trans-Golgi network, there are reports indicating a possible role for Arl1 in ion homeostasis in yeast.

Currently there is no direct assay to measure the activation of Arl1 GTPases.

NewEast Biosciences Arl1 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Arl1-GTP, but not Arl1-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti-Arl1-GTP monoclonal antibodies can also be used to monitor the activation of Arl1 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Arl1 Activation Assay Kit provides a simple and fast method to monitor the activation of Arl1. Each kit provides sufficient quantities to perform 20 assays.

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Assay Principle

NewEast Biosciences Arl1 Activation Assay Kit bases on the configuration-specific anti-Arl1-GTP monoclonal antibody to measure the active Arl1-GTP levels, either from cell extracts or from in vitro GTPγS loading Arl1 activation assays. Briefly, anti-active Arl1 mouse monoclonal antibody will be incubated with cell lysates containing Arl1-GTP. The bound active Arl1 will then be pulled down by protein A/G agarose. The precipitated active Arl1 will be detected by immunoblot analysis using anti- Arl1 rabbit polyclonal antibody or anti-Arl1 mouse monoclonal antibody.