E tag polyclonal antibody
產品名稱： E tag polyclonal antibody
英文名稱： E tag polyclonal antibody
產品編號： PAB12765
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptides of E tag.
- Immunogen:
- A synthetic peptide corresponding to E tag.
- Sequence:
- GAPVPYPDPLEPR
- Host:
- Rabbit
- Specificity:
- This antibody recognizes E-tagged fusion proteins.
- Form:
- Liquid
- Storage Buffer:
- In PBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane.
Campbell AM, Kessler PD, Fambrough DM.J Biol Chem. 1992 May 5;267(13):9321-5.
- Applications
- Western Blot (Transfected lysate)
- The whole cell lysate of NIH/ 3T3 with over-expressed E-Tag fused recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with E tag polyclonal antibody (Cat # PAB12765) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide.