SPECIFICATION SHEET

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Catalog #:?????????????????? L1C00401 ??????????????Lot #:???????????????????????????? C040901

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Description: ?????????????????? MAb to PSA

??????????????????????????????????????????????? Monoclonal Antibody to total Prostate Specific Antigen (tPSA)

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Specificity:???????????????? The total Prostatic Specific Antigen (tPSA) is a tumor associated antigen with a molecular weight of 34Kd.? This antigen is found in normal prostatic epithelial lining cells of the ducts and acini, as well as, in primary and metastatic prostate carcinoma cells.

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Clone:???????????????????????? ML00P01

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Host Animal:????????????? Mouse.? Hybridization of P3-X63-Ag8.653 .myeloma cells with spleen cells from Balb/c mice.??

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Isotype: ???????????????????? IgG₁, ???????????Source: ???????????????? Ascites

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Immunogen:?? ???????? PSA prepared from human seminal fluid.

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Format:????????????????????? Purified, Liquid

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Purification:? ?????????? >90% pure (SDS-PAGE).? Protein A chromatography.

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Concentration: ??????????? 2mg/ml? (0.7×OD280nm)

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Affinity Constant:? ? 5 x 10^-9 mol/l.

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Buffer:??????????????????????? 10mM Sodium Phosphate, 0.15M Sodium Chloride, pH 7.4

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Preservative: ??????????? none

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Applications: ??????????? Immunohistochemistry on frozen or paraffin sections.? Each laboratory should determine an optimum working titer for use in its particular application.

Recommended antibody pair for immunoassay:

??????????????????????????????????? Capture??????????????????????????????? Detection

??????????????????????????????????? L1C00401??????????????? L1C00402

Storage:????????????????????? Upon receipt, aliquot and store at <–40°C.? Avoid multiple freeze/thaw cycles.? If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used.