MBP polyclonal antibody
產品名稱： MBP polyclonal antibody
英文名稱： MBP polyclonal antibody
產品編號： PAB10344
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against recombinant MBP (Maltose Binding Protein).
- Immunogen:
- Recombinant protein corresponding to MBP (Maltose Binding Protein).
- Host:
- Rabbit
- Specificity:
- This polyclonal anti-MBP epitope tag antibody detects over-expressed proteins containing the MBP epitope tag.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:4000-1:16000)
Western Blot (1:3500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Recombinant protein)
- MBP polyclonal antibody (Cat # PAB10344) detects MBP-tagged recombinant proteins by western blot.
MBP epitope tag polyclonal antibody at 0.5-1.0 ug/mL was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag.
A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 2500 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
LICOR's Odyssey? Infrared Imaging System was used to scan and process the image.