基本信息

產品名稱HRP 標記的鏈霉親和素英文名稱SA-HRP規格或純度Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed.種屬Streptomyces avidinii序列MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLG STFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWT VAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVG HDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ蛋白長度Full length proteinCarrier Free1% BSA偶聯標記HRP來源Native

相關屬性

特點與好處This product was prepared by highly purified Streptavidin and ultrapure horseradish peroxidase (RZ > 3.0). Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed. Streptavidin-horseradi應用WB, Dot, ELISA, ICC, IHC-Fr, IHC-P物理外觀Liquid儲存緩沖液PBS (pH 7.4) buffer containing 1% BSA, glycerol, and 0.05% ProClin? 300 as a preservative.防腐劑0.05% ProClin? 300濃度Streptavidin Concentration: 1 mg/mL儲存溫度-20°C儲存,避免反復凍融運輸條件超低溫冰袋運輸穩定性與儲存Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle and protected from prolonged exposure to light. Prepare working dilution on day of use.

常規信息

英文別名SA-HRP (HRP-labeled Streptavidin); Streptavidin-Horseradish Peroxidase; SAv-HRP; SA V1; SA V2; streptavidin; Streptavidin V1; Streptavidin V2別名SA-HRP (HRP-labeled Streptavidin); Streptavidin-Horseradish Peroxidase; SAv-HRP; SA V1; SA V2; streptavidin; Streptavidin V1; Streptavidin V2

一般描述

背景

鏈霉親和素是一種來自streptomyces avidinii菌的非糖基化蛋白。鏈親和素同源四聚體對生物素具有特別高的非共價結合親和力。當與酶（如辣根過氧化物酶或堿性磷酸酶）形成偶聯物并與比色或發光底物發育系統結合時，鏈霉親和素與生物素化抗體在許多應用中得到廣泛應用，包括Western blot、ELISA、ELISPOT、免疫細胞化學和免疫組化。

推薦工作濃度

WB : 1:10000-1:100000

ELISA: 1:1000-1:10000

避免使用含有生物素的稀釋液，試劑不得含有疊氮化鈉，疊氮化鈉會抑制辣根過氧化物酶的活性。實際試驗操作過程中可根據具體實驗情況適當調整Streptavidin(HRP)的稀釋比例。

Background

Streptavidin is a non-glycosylated protein from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.

Recommended Usage：

WB: 1:10000 -1:100000

ELISA: 1:1000-1:10000

Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

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WB-Streptavidin (HRP) (np156148)

WB-Streptavidin (HRP) (np156148)

Various whole cell lysates were subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:10000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 10s.

ELISA-Streptavidin (HRP) (np156148)

ELISA-Streptavidin (HRP) (np156148)

ELISA results of Streptavidin (HRP) (np156148) tested against Biotin-BSA. Each well was coated in duplicate with 1 ng of Biotin-BSA.

WB-Streptavidin (HRP) (np156148)

WB-Streptavidin (HRP) (np156148)

HT-29 whole cell lysate was subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:5000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 8s.