Product: Anti-c-myc mAb, clone 9E10
Cat. No.: MC-311 (100 μg)
Background:
Clone 9E10 can be used to detect the protein on
western blot or to immunoprecipitate the
recombinant protein when doing binding studies
with transfected cells. Balb/C mice were
immunized with the synthetic peptide
EQKLISEEDL coupled to carrier. The resultant
hybridomas were selected for reactivity with the
immunogen by ELISA and with the antigen by
western blot.
The native c-myc protein is very labile. Lysis
buffers should be supplemented with protease
inhibitors if detection of intact c-myc is the
objective.
Specificity:
Clone 9E10 anti-c-myc specifically recognizes
the 10-amino acid epitope of human c-myc
amino acids 410-419.
Ig Isotype:
Mouse IgG1
Format:
100 μg of purified monoclonal antibody at
1 mg/mL in 0.02M sodium phosphate, pH 7.5,
0.15M sodium chloride, 50% glycerol, and 3 mM
sodium azide. .
Storage:
Store at -20°C. Aliquot to avoid freeze/thaw
cycles.
Applications and Suggested Dilutions:

ELISA: Use at 1-5 μg/mL.

Immunofluorescence

Immunohistochemistry

Immunoprecipitation

Western blot: Use at 1-5 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.