Product: Anti-DNA Polymerase ? mAb, clone 18S
Cat. No.: MC-370 (100 μg)
Background:
DNA polymerase ? has an 8 kDa amino-terminal
ssDNA binding domain with deoxyribose
phosphodiesterase activity and a 31 kDa
carboxy-terminal domain with
nucleotidyltransferase activity. Mammalian DNA
polymerase ? is constitutively expressed in
cultured cells, but levels can be up-regulated by
treating cells with DNA-alkylating agents (eg.
methyl methanesulfonate). DNA polymerase ?
fills single nucleotide gaps in DNA produced by
the base excision repair pathway of mammalian
cells.
Specificity:
Monoclonal antibody recognizes the 39 kDa
DNA Polymerase ?. Shows no effect on the
polymerase activity of ?-pol.
Positive Control:
HeLa cells.
Cellular Localization:
Nuclear
Epitope:
Not determined
Species Reactivity:
Human, bovine, mouse, rat, hamster, and
Xenopus. Others not known.
Ig Isotype:
Mouse IgG1
Immunogen:
Rat DNA Polymerase ? protein
Format:
500 μL of 200 μg/mL monoclonal antibody
purified from ascites fluid by Protein G
chromatography. Prepared in 10 mM PBS, pH
7.4, with protein stabilizer and 0.09% sodium
azide.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Functional Activity: Effect on Polymerase
Activity, Non-inhibitory.

Western blot: Use 1-2 μg/mL for 2 hrs. at RT.

Immunoprecipitation (Denatured verified) Use
protein G; Ab at 2 μg/mg protein lysate.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.