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Mouse Erythropoietin Receptor ( EPOR / EPO-R ) Protein
 
 
 
EPOR / EPO-R Protein Price Inquiry ( Available Sizes )
 
 
 
500μg:? 詢價;
 
≥1mg?大包裝: ?詢價
 
 
 
EPOR / EPO-R蛋白產品信息
 
 
 
 
別名:
 
 
Epor
 
 
 
 
表達方式:
 
 
A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3) (Met 1 - Pro 249) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus
 
 
 
 
種屬:
 
Mouse
 
 
 
表達宿主: 
 
Human Cells
 
 
 
 
EPOR / EPO-R蛋白質控指標
 
 
 
 
純度:
 
> 85 % as determined by SDS-PAGE
 
SDS-PAGE:
 
 
EPOR protein
 
 
 
 
內毒素:
 
< 1.0 EU per μg of the protein as determined by the LAL method.
 
 
 
穩定性:
 
Samples are stable for up to twelve months from date of receipt at -70℃
 
 
 
預測N端:
 
Ala 25
 
 
 
分子量:
 
 
The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 473 amino acids with a predicted molecular mass of 52.8 kDa. As a result of glycosylation, rmEPOR migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions
 
 
 
 
緩沖液:
 
 
Lyophilized from sterile PBS , pH 7.4
 
 
 
 
 
EPOR / EPO-R蛋白使用指南
 
 
 
 
儲存方法:
 
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
 
 
 
 
 
Reconstitution:
 
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 
 
 
 
EPOR / EPO-R蛋白相關產品和研究主題
 
 
相關研究領域:
 
Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Endothelial Cell Marker>>EPOR
 
Immunology>>Cells of the Immune System>>Red Blood Cells>>EPOR
 
蛋白:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info.------CLICK!
 
Cat No
 
 
 
EPOR
 
Human
 
EPOR/Fc protein, Recombinant
 
10707-H02H
 
 
 
EPOR
 
Mouse
 
EPOR/Fc protein, Recombinant
 
50031-M03H
 
 
 
EPOR
 
Mouse
 
EPOR protein, Recombinant
 
50031-M08H
 
 
 
 
抗體:
 
 
EPOR / EPO-R蛋白背景綜述
 
 
Mouse Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis , and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR is a type 1 single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. Mouse EPOR contains a tryptophan-serine-X-tryptophan-serine (WSXWS) motif and one copy of a cytoplasmic immunoreceptor tyrosine-based inhibitor motif (ITIM) motif, and shares approximately 80% amino acid sequence identity with the human EPOR. Mouse EPOR is expressed in relatively mature erythroid progenitor cells and in EPO-responsive erythroleukemia cells. EPO binding to EPOR triggers auto- or trans-phosphorylation of a protein-tyrosine kinase JAK2 bound to the receptor cytosolic (CT) domain, and subsequently JAK2 activates a variety of intracellular signal substrates including Ras/MAPK, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to function in erythroid cell survival through preventing the PCD, and is also suggested to play a neuroprotective role for its expression in mammalian brains.
 
 
參考文獻
 
 
 
Jones, S.S. et al., 1990, Blood. 76: 31-5.
 
Liboi, E. et al., 1993, Proc. Natl. Acad. Sci. USA. 23: 11351-5.
 
Yoshimura, A. and Arai, K. 1996, Oncologist. 1: 337-9.
 
Kawakami, M. et al., 2001, J. Biol. Chem. 276: 39469-75.
 
Wojchowski, D.M. et al., 1999, Exp. Cell. Res. 253: 143-56.
 
Witthuhn, B.A. et al., 1993, Cell. 74: 227-36.
 
 
 
 
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